The medium was then removed and 100 μL of DMSO was added to dissolve the formazan crystal. Description 1. Test samples were measured according to the manufacturer’s instructions using a spectrophotometer SpectraMax M4 (Molecular Devices, Sunnyvale, CA). Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. Parameter SpectraMax iD5 SpectraMax i3x. Background-subtracted data values (mean +/- SD) are graphed. Danaher Inc spectramax m series Spectramax M Series, supplied by Danaher Inc, used in various techniques. 5 pM 2. Cytosolic [K +] was then monitored using a SpectraMax M4 spectrofluorometer (San Jose, CA, USA) by loading AM at 37 ℃ for 40 min with 12 µM APG-2. Hexokinase reagent (Eagle Diagnostics) was added and the reaction absorbance was read at 340 nm using a SpectraMax M4 multimode plate reader (Molecular Devices). The Dagda Lab is a mitochondrial biology-oriented research group located at the University of Nevada, Reno School of Medicine that currently spearheads basic and translational research projects supported. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode. SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise: Trinean: DropSense96: AMBIENT TEMPERATURE STORAGE: Liconic: LPX 220 Hotel: LPX 440 Hotel: Thermo Fisher Scientific: Cytomat 10 Hotel: Cytomat 2 Hotel: Cytomat 24 Hotel: Cytomat 8 Linear Hotel: Cytomat 9 Hotel (MPH) BAR CODE. Dumont3, Matthias S. com. Background-subtracted data values (mean +/- SD) are graphed. MTT values at day 3 were normalized to day 0. 7 cells. TYPICAL SAMPLE VALUES Sensitivity: The calculated minimal detectable dose (MDD) is 15. 2. spr SpectraMax Plus 384. When using a SpectraMax M3 Reader, the GxP audit trail incorrectly reported the instrument as a SpectraMax M4 Read er. To investigate the pharmacokinetics of HSA–CBT-NPs, 10 male Sprague-Dawley rats (mean±SD=250±20A SpectraMax M4 microplate reader was purchased from MeiGu Molecular Co. In. Dissolve FITC-dextran in PBS at a. All configurations. However, little is known about the regulatory mechanisms of the degradative pathway. 3. To avoid injury, it is recommended that two. In luminescence mode, if you are specifying a wavelength for the read, you can select an emission bandwidth of either 15 nm or 25 nm. All configurations. For a complete list of the instruments supported by this release of the SoftMax. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The SpectraMax M2 and M2e are versatile and reliable. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email [email protected]. 2. When a feature or capability applies to only certain readers, this exception is noted. SpectraMax® Paradigm® Multi-Mode Detection Platform. Category 2 and 3 microplate readers use gradient heating as well. Serving Victoria's renovation and construction needs. 9000-0133 SpectraMax®MousePad 9000-0972 Packageof5samplemicroplates 2400-2464 DustCover 5000505 CEDeclarationofConformity 5019680 Card,UserGuideDownload The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Transfer plate to SpectraMax M5 with excitation monochromator set to 455 nm with 10 nm bandwidth, emission monochromator set to 525 nm with 10 nm bandwidth, and 515 nm cutoff filter. The samples were centrifuged to pellet the remaining intact erythrocytes, and supernatants were collected. The optical density was measured using a microplate reader (Spectramax M4, Molecular Devices, San Jose, CA, USA) at 570 nm. SpectraMax M4 reader had identical performance specifications to the SpectraMax M5 reader for absorbance and fluorescence detection. The optical absorbance of the plate at 450 nm was measured using a multifunctional microplate reader SpectraMax M4 (Molecular Devices, LLC, San Jose, CA, USA). Roelli1, Amandine Stoss1, Rebecca A. 23, SpectraMax M3, M4, M5, and M5 e Multi-Mode Microplate Readers 0112-0115 F 23 PathCheck Pathlength Measurement Technology and Interfering Substances Any material that absorbs in the 900 nm to 1000 nm spectral region could interfere with PathCheck Pathlength Measurement Technology measurements. book Page 1 Wednesday, July 7, 2010 2:09 PM. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. The following document is intended to demonstrate setup of this instrument. 73. A microplate reader (SpectraMax M4; Molecular Devices) was used to measure the absorbance (Abs) at 545 mM and the hemolysis rate was calculated as follows: (Eq. Methods for the accurate and rapid screening of IVT conditions will facilitate RNA polymerase engineering, promoter optimization, and screening for new transcription inhibitor drugs. Colony Formation Assay. 05 mg mL −1. , contact Molecular. After incubation at 37 °C for 3 h, the fluorometric detection of cleaved AFC and chromogenic product of pNA was performed on a Multimode Plate Reader (Spectramax M4, Molecular devices, USA) using a 400 nm excitation filter and a 530 nm emission filter for detection of caspase 3 and 410 nm wavelength for detection of caspase 9 using. C. Microplate Readers include the M3, M4, exception is noted. Tris solution and the fluorescence of the solution was measured using a fluorometer (SpectraMax M4, Molecular Devices). Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. Fluorescence Microplate Reader Comparison Chart. The medium was then removed and 100 μl of DMSO was added to dissolve the formazan crystal. 316 grating motor driver faulty. No. Published Dec 16, 2020 | Updated Jan 25, 2021 Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, Gemini XPS reader, SpectraMax M5/M5e reader, SpectraMax. VIEW DETAILS. Page: 1/6. /525 em. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Plot the fluorescence intensity versus the cell seeding number. 4) and cells were stained with 150 nM TSQ for 1 min. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. . Absorbance was read at 570 nm with SpectraMax M4 microplate spectrophotometer (Molecular Devices, CA). • SpectraMax® M3/M4/M5/M5e Multi-Mode Microplate Readers • SpectraMax® Paradigm® Multi-Mode Microplate ReaderThe medium was replaced with CM from the macrophages of the different groups and then incubated for 48 or 72 h. FlexStation™ III. The reaction systems were cultured in 37°C for 1 h, and the OD405 values were read on SpectraMax M4 (Molecular devices). $1,250. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. . Exhibit with us {{>currencyLabel}} Back. All configurations. 1. For cell cultures, the medium was removed 24 h after initiation of drug stimulation and frozen for subsequent analysis, with no need for sample dilution. Microplate Reader Shelf. Standard curves for quantifying samples were generated by using serial dilutions of BSA from 0 to 0. Data are presented as mean values ± standard. Supplier: Molecular Devices. SpectraMax® M4). Resolution: In the GxP audit trail, the SpectraMax M3 and M4 Readers are now identified correctly. iD3, iD5, i3, i3x, M3, M4, M5, M5e, L, and FlexStation 3 readers. Background-subtracted data values (mean +/- SD) are graphed. Lysosomal proteolytic activity. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). SpectraMax® M4. GSA System Includes: SpectraMax M4 Multi-Mode Spectrophotometer with Tunable. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email [email protected] number of moles of FITC conjugated to OVA was determined using UV–vis spectroscopy (SpectraMax M4, Molecular Devices Inc. 383 inches whereas the maximum allowed is 5. For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. . D 1 1. Western blot apparatus (BIO-RAD Laboratories). The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. Cell viability (%) was calculated as the ratio of the number of living cells in treated samples to that of the control [15,30]. SigmaPlot (Systat Software, Inc. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, Gemini EM reader, SpectraMax M5/M5e reader, FlexStation 3 reader, SpectraMax M4 reader Which is better: top-read or bottom-read? You can use either. alamarBlue reagent volume in a different spheroid microplate formatA SpectraMax M4 microplate reader (Molecular Devices, LLC, Sunnyvale, CA, USA) was used to measure the absorbance at a wavelength of 450 nm. Calpain activity assay The cells were treated with different drugs for 24 hours and were resuspended in 100 μL extraction buffer and homogenized by pipetting. StakMax Handling Stacker Base Unit. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. Molecular Devices’ unique SpectraDrop™ MicroVolume Microplate offers the highest throughput solution for low volume measurement available on the market today. Brand: Molecular. Specifications LUMINESCENCE PHOTOMETRIC PERFORMANCE Detection limit (384-well microplate) 10 amol/well alkaline phosphatase, 200 µL Wavelength range 250–850 nm TIME. Call 516-939-0888 to check current. The SpectraMax M4 reader had identical performance specifications to the SpectraMax M5 reader for absorbance and fluorescence detection. book Page 1 Wednesday, July 7, 2010 2:09 PM SpectraMaxM3,M4,M5,andM5eMicroplateReaderUserGuide 8 0112-0115G CAUTION!Useoforganicsolventscancauseharmtotheopticsintheinstrument. Measurement of Mitochondrial Membrane Potential by JC-1. In the competitive field of drug development where timely go/no-go decisions must be made to bring effective class-leading drugs to market, it is vital to work with a CRO that can provide highly. SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise: Trinean: DropSense96: AMBIENT TEMPERATURE STORAGE: Liconic: LPX 220 Hotel: LPX 440 Hotel: Thermo Fisher Scientific: Cytomat 10 Hotel: Cytomat 2 Hotel: Cytomat 24 Hotel: Cytomat 8 Linear Hotel: Cytomat 9 Hotel (MPH) BAR CODE READING: FluidX: Perception (Vial. Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. All luciferase assays were conducted in CORNING ® TypeI Collagen Coated 96-Well Cell Culture Clear Bottom White plates. Five hours prior to when permeability will be measured, gavage mice with 0. Fifteen different automated tests are provided: • Background noise • Background spike • Lower limit of detection • Crosstalk • Linearity • Relative luminescence units (RLU) • Kinetic noise (low signal) 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuide SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. Three kits covering a wide range of dsDNA concentrations. . Preconfigured SoftMax ® Pro Software protocols (available at softmaxpro. 37. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. The incubation system pre-incubated at 37°C for 3 min minutes and 2 μL fluorescent substrate FD (5 μM, final concentration) was added for fluorescence analysis in fully automated fluorescent microplate reader (SpectraMax M4, Molecular Devices) (Wang et al. Our absorbance plate readers feature our PathCheck Sensor. Products : SpectraMax M2/M2e reader, SpectraMax Plus 384 reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, SpectraMax M3 reader Can you recommend disposable cuvettes for use in the microplate readers? Molecular Devices® does not recommend any specific disposable cuvette brands. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. Spectramax M Series, supplied by Danaher Inc, used in various techniques. /525 em. 発光モードに対応した機種(SpectraMax M5e/M5/M4/M3)では、発光モード専用のフォトンカウント検出器も搭載しました。 蛍光に特化した検出器を流用するのではなく、専用の光学系を採用することで、発光、TR-FRET、蛍光偏光アッセイにおいても専用機に匹敵す. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating. spr SpectraMax iD3 SpectraTest ABS2 v1. 6 mm). Multi-Mode Microplate Readers. Following digestion, 200 μl from each sample was pipetted into a 96-well plate, and samples were measured in duplicate at 485- and 525-nm excitation and emission, respectively (SpectraMax M4, Molecular Devices). At sacrifice, serum was isolated from mouse total blood and maintained at –80 °C. Click Read. Instruments and Settings for Fluorescence Read-out (Other Fluorescent Assays) FLUOstar Omega, VANTAstar, CLARIOstar, PHERAstar, PolarStar Optima. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. Table 2. 1. Products : SpectraMax M4 reader, FilterMax F5 reader, SpectraMax Paradigm reader, SpectraMax M5/M5e reader Do you know any applications for TRF/HTRF that differ from Fluorescence Polarization? TR-FRET assays are well-suited for protein:protein interactions. The viability of cells was determined by using SpectraMax® M4 Multi-Mode Microplate Reader (Molecular Devices, Sunnyvale, CA, USA) at 570 nm absorbance . Data was calculated subtracting OD440 recorded with the final OD600 values. SpectraMax® ABS Plus. Italic and boldface type are used for emphasis. Fluoresbrite® fluorescent nanoparticles were analyzed by spectrofluorimetry where particles were excited at 441 nm wavelengths and emission intensity was recorded at 486 nm wavelengths. 1% CF3COOH) = 5/95 (v/v) to 95/5 (v/v); flow rate:The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. The absorbance at 550 nm was recorded using a SpectraMax M4 microplate reader (Molecular Devices) following solvation of formazan with 100 μL DMSO. FD-4 concentrations in plasma were analyzed in duplicates using a spectrophotometer (SpectraMax M4, Molecular Devices, San Jose, CA, USA) with excitation λ 485 nm and emission λ 535 nm. 5 mL of creatinine, FD-4, and rhodamine70 dissolved in. SpectraMax M3 Reader SpectraMax M4 Reader SpectraMax M5 Reader SpectraMax M5e Reader 5 B > 4 4 5 B 5 : F 8 8 > 3 ; > I 5 = 8 5. Lastly, the TMB substrate (Beyotime) was added, and absorbance was measured at 450 nm by a microplate reader (SpectraMax M4). Diclofenac potassium at non-toxic concentration on U87-MG was used as positive control. Detection modalities are shown in Table 1-1. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. 5 pM 384-well (50 µL) 6 pM 10 pM 1536-well (8 µL) 19 pM 25 pM 0200-7015 (LUM 384) Optimized Guaranteed* 96-well (200 µL) 1. VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. If using the StakMax and a SpectraMax reader together, the time specifications for the movement of the. +1 250-415-0785. The chamber contains springs that position the cuvette in the proper alignment for a read. SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. 2 only). Preconfigured SoftMax® Pro Software protocols (available at softmaxpro. For the SpectraMax M3/M4/M5 readers - use the Purple Assay Plate adapter for Top Read only. The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. 303 unable to cal dark current. 304 signal level saturation. Mitochondrial respiration using high-resolution respirometry . Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. Italic and boldface type are used for emphasis. 7. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Place the instrument on a level surface, away from direct sunlight, dust, drafts, vibration, and moisture. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Briefly, 4T1 cells were seeded (5000 cells/well) in a 24-well plate. Products : SpectraMax M3 reader, SpectraMax M4 reader, SpectraTest Validation Plates LM1 , FlexStation 3. Highly selective for dsDNA over ssDNA or RNA. With optimized reagents, option to operate the. The aim of the present study was to investigate the preventive effects of. instruments you must first insert an adapter plate. The absorbance of formazan was detected at 540-nm wavelength with a reference wavelength at 650 nm for background using a SpectraMax M4 plate reader (Molecular Devices). In this application note, we demonstrate the measurement of luciferase expression in CHO-K1 cells using the SpectraMax Glo Steady-Luc Reporter Assay Kit, which affords long-lasting luminescence signals. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. , 2011). Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. MIC assay: After diluting colloidal nanoparticle solutions of varying concentrations in TSB, they were injected onto a 96-well plate with a strain of bacteria at a concentration of 10 6 CFU/mL. 0397 in x 3. For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. Background-subtracted data values (mean +/- SD) are graphed. The ability of the liposome to be internalized into cell was assessed using cell culture studies 45-47. 7°C and the bottom to 36. Serum C-reactive protein (CRP) was quantified using the Mouse C-Reactive Protein/CRP Quantikine ELISA Kit. (Fair Lawn, NJ, USA). SpectraMax® L (software version 5. 7% of the total test aerosol mass) was not included in the collection analysis. The content was vortexed for 1 min and incubated in the dark for 20 min at 25°C. ® reader to the computer. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. 313 reference gain check fail. Add To My List; Create New List; Quickview. Members of the Sphingomonadales are renowned for their ability to degrade polycyclic aromatic hydrocarbons (PAHs). Residual. 95. Examples: “Press carefully to engage,” “ Do not press down. The absorbance was determined by a microplate reader (SpectraMax M4, USA) at 405 nm. FlexStation ®, SpectraMax. 306 plate air cal fail,low light. The percentage of viability of each treatment group in different. Fluorescence intensities of each well were examined by a microplate reader (SpectraMax M4, Molecular Devices, CA, USA) with 485-nm excitation and 525-nm emission wavelengths. After 48 h, the optical density (OD) was measured at 450 nm using the microplate reader (SpectraMax M4). SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. Page 17 Chapter 2: Set Up the StakMax Microplate Handler Microplate Handler Mounted Baseplate Connection This procedure provides instructions to connect the microplate handler to the following instruments: Gemini EM Gemini XPS SpectraMax M2 SpectraMax M2e SpectraMax M3 SpectraMax M4 SpectraMax M5 SpectraMax M5e SpectraMax. e. Living cells were then incubated with 100 µl of medium assay provided by the assay kit. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. TEM. Consult Molecular Devices's entire SpectraMax M series catalogue on MedicalExpo. 11. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. 光验证板,用于验证 SpectraMax M3、M4 和 M5/M5e 多功能读板机的光 学性能表现。 SoftMax Pro 内置 140 多种预存实验方法可以直接调用,可以进行数 据获取和个性化的数据分析;强大的图标绘制工具和文本编辑功 能,可以方便的进行各种数据结果的展示。 完整的. In the M2, M5 and Plus 384, the light. Large Molecule Bioanalysis. The biofilm mass was quantified through the measurement of optical density of the sample at 570 nm using a spectrophotometer (SpectraMax® M4 Multi-Mode Microplate Reader, Molecular Devices). The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. Background-subtracted data values (mean +/- SD) are graphed. Finally, the optical density was evaluated using SpectraMax M4 plate reader (Molecular Devices, San Jose, CA, USA) at 450 nm. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. S. 9000-0756. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Samples (2 mg) were mixed with potassium bromide in a 1:100 ratio before the analysis. For more detailed information and technical support of Molecular Devices instruments or software,The sample solution was added to DPPH. , cyan fluorescence at 458 ex. Multi-Mode Microplate Readers including the M3, M4, M5, and M5. Absorbance was measured at 570 nm using a multimode reader (Spectramax M4, USA) (Kalvala et al. SpectraMax® Paradigm® Multi-Mode Detection Platform. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v2. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuide SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. The IC50 curves were calculated using variable slope for log (inhibitor) vs. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. Plus 384 ABS1 Location: Reader Validation-Plate Abs A reminder about recertification was added. Molecular Devices SpectraMax ® M3/M4/M5/M5e Multi-Mode Microplate Readers + + Molecular Devices SpectraMax. 2. Choose from a three- (M3), four- (M4), orThe absorbance of the biofilm was measured at 595 nm using a microplate reader (SpectraMax M4) and the results were expressed as percentage changes with respect to the control (without compounds). August 16, 2017. To quantify F-CHP fluorescence, stained samples were digested in 500 µl of 1 mg/ml proteinase K in water for 3 hours at 60 °C. Establishment of mouse orthotopic xenograft bladder tumor model. Shop our huge selection of OEM, MRO, construction, industrial, and safety products. e. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. book Page 1 Wednesday, July 7, 2010 2:09 PMLuciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. Also, when using a SpectraMax M4 Reader, the GxP audit trail incorrectly re ported the instrument as a SpectraMax M3 Reader. . The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. Like all. After 7 days of culture, the colonies generated. 305 reference level saturation. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date: 5/20/2019 9:24:38 AM SpectraMax M4 Multi-Mode Microplate Reader. org) with optimized instrument settings (Table 1) were used to generate and analyze data. Measurements of temperature increase (ΔT) during the exposure of MNP/AMF hyperthermiaThe culture medium was removed, and the blue product was dissolved in DMSO (100 μl). MEK Inhibition Induces Therapeutic Iodine Uptake in a Murine Model of Anaplastic Thyroid Cancer Oussama ElMokh1, Vincent Taelman2, Piotr Radojewski2, Matthias A. On day 2, in the morning weight the mice. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. Luminescence was immediately detected by the SpectraMax M4 Microplate/Cuvette Reader (Molecular Devices, San Jose, CA, USA). . Sandwich ELISA - Human TNF alpha ELISA Kit, Fluorescent (ab229399) To learn more about the advantages of recombinant antibodies see here. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence polarization, and time-resolved. 0 Micromoles per Joule, ensuring the highest light output using the least amount of electricity. Ninety microliters of 2-chloro-4-nitrophenyl-α- d -maltotrioside substrate dilutions from 5000 to 1. The MTT reagent was incubated with cells for 4 h. SpectraMax M3, M4, M5, and M5 e. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. Technical specifications Detection limit for ATP Glow 0200-7012 (LUM) Optimized Guaranteed* 96-well (200 µL) 1. The plate reader. SpectraMax M3 Multi-Mode Microplate Reader with 1-Year Service Contract. AquaMax® 2000. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. Cell viability was calculated using the formula as follows: (OD450 KO HepG2 -OD450 blank /OD450 WT HepG2 -OD450 blank ) × 100%. Molecular Devices SpectraMax® series microplate readers. We therefore gathered salient. Operated from(A) ROS levels and (B) NAD+/NADH ratios in rotenone-pretreated young HDFs. Hemoglobin release from erythrocyte lysis was detected by measuring the optical density (OD) of the supernatant at 412 nm using a microplate reader (SpectraMax M4 from Molecular Devices, or Tecan SPARK, from Tecan, Männedorf,. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. alamarBlue reagent volume in a different spheroid microplate formatAfter incubating for 10 min at room temperature, the absorbances of standards and samples were measured at 450 and 590 nm, 12 using a Spectramax M4 spectrophotometer (Molecular Devices). Finally, we have two New Brunswick Excella E24 shaking incubators, one with refrigeration capabilities and a Thermo Sciences -80˚C freezer. At sacrifice, serum was isolated from mouse total blood and maintained at –80 °C. The absorbance change at 620 nm was measured by a SpectraMax M2 Microplate Reader (Molecular Devices). The polysaccharide HPA with different concentrations (0, 25, 50, and 100 μg/mL) was added and incubated with cells for 24 h. SpectraMax® M4 Multi-Mode Microplate Reader MLDVM4To quantify F-CHP fluorescence, stained samples were digested in 500 μl of proteinase K (1 mg/ml) in water for 3 hours at 60°C. Then cells were collected, and the caspase-3 expression level was detected with the caspase-3 assay kit. Following digestion, 200 µl from each sample was pipetted into a 96-well plate, and samples were measured in duplicate at 485 and 525 nm excitation and emission, respectively (SpectraMax M4, Molecular Devices). of a 96 well-plate and cultured for 24h at 37 °C. 2. The filtered samples were transferred to a 96-well plate and the fluorescence was measured using a plate reader (Spectramax ™ M4, Multi detection Reader) at excitation and emission wavelengths of λex 339 and λem 390 nm, respectively. At 48 h after transfection, the Nano-Glo Live Cell Assay reagent was added and the luciferase activity in the medium and cells were measured using a SpectraMax M4 (Molecular Devices, San Jose, CA, USA). Cells were seeded at 5000 cells well −1 on 96-well plates and allowed to grow for 24 h before treatment. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. 1, BIO-RAD Laboratories). The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. SpectraMax M2/M2e reader: TRUE: Molecular Devices: Aquamax DW4 washer: TRUE: Molecular Devices: SpectraMax M4 reader: TRUE: Molecular Devices: SpectraMax M5/M5e reader: TRUE: Molecular Devices:. > spectramax m4 microplate reader. Dettmer4, Wayne A. Products : SpectraMax M2/M2e reader, SpectraMax Plus 384 reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, SpectraMax M3 reader Where can I find UV/Vis Plastic Disposable Cuvettes? Some Plastic Disposable UV (300 nm +) and VIS Cuvette sources: NOTE: Some disposables with channel sides (as opposed to full. . SpectraMax ABS/ABS Plus SpectraMax i3/i3x MiniMax SpectraMax iD3/iD5 Spectramax 190 EXT Spectramax 340PC/384 Spectramax Plus 384 Spectramax L Spectramax M2 Spectramax M2e Spectramax M3 Spectramax M4 Spectramax M5 Spectramax M5e Spectramax Mini Versamax EXT Vmax Microplate Washers;. Fastenal is the largest fastener distributor in North America. The enzymatic reaction was triggered by addition of luminol and the chemiluminescence signal was monitored at 37 °C using a microplate reader SpectraMax M4 (Molecular Devices, Sunnyvale, CA, USA), with one acquisition every 2 s for 300 min. The cleaved substrate was excited at 356-nm, and the fluorescence emissions were detected. Description 1. Then, the fluorescence intensity was quantified by using a spectrophotometer (Spectramax M4, MolecularDevices, San Jose, CA, USA) at λ ex: 579 nm and λ em: 599 nm. TSQ was dissolved in Lock’s buffer (pH 7. D 1 1. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-Mode MicroplateReader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5e. 1. The SpectraMax M5e reader offers the additional benefit of being certified for Cisbio Bioassays HTRF® technology. F1 SpectraMax Plus384andABSPlus: Certificate Value – 2≤ Average Peak Value≤ Certificate Value + 2 SpectraMax M2,M2e,M3,M4,M5,andM5e: Certificate Value – 3≤ Average Peak Value≤ Certificate Value + 3 Photometric Accuracy F2, F3, F4 AverageOD=Certificate Value± (Certificate Value(0. Description 1. SPECTRAMAX 190 reader: TRUE: Molecular Devices: SPECTRAMAX 340PC/340PC 384 reader: TRUE: Molecular Devices. 0 compensates for most changes in environmental temperature or pressure. Products : SpectraMax M3 reader, SpectraMax M4 reader, SpectraTest Validation Plates FL1, Gemini EM reader, Gemini XPS reader, SpectraMax M5/M5e reader My SpectraTest FL one plate is failing for LLD for the wet test. ® reader to the computer. High tolerance for contaminants. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, SpectraMax M4 reader, SpectraMax M3 reader MTT reduction - a tetrazolium-based colorimetric assay for cell survival and proliferation (Maxline Application Note #5) (PDF) Summary: The MTT assay measures cell respiration and the amount of formazan. Introduction. 1 mg·mL −1 (Sigma-Aldrich, USA) as described above and fluorescence intensity at λ ex = 530 nm and λ em = 590 nm was detected through a microplate reader (Spectramax M4, Molecular Devices, San Jose, CA, USA). Steady-state kinetics measurements were performed at 25°C in 96-well plates (Corning) using a SpectraMax M4 spectrophotometer (Molecular Devices) in PBS buffer. l. Firefly luciferase, a 61 kD monomeric protein, is especially attractive to many researchers because of its high sensitivity, wide linear detection range and extremely low background due to the absence of endogenous luminescent activity in mammalian cells. All experiments. Images were taken using a fluorescence microscope (Nikon 80i, Japan). The adherent cells were incubated in 100 μl of lysis buffer on ice for 20 min. The ELISA kits (Lianke Bio, China) were used to detect the concentration of specific cytokines. Other technologies such as imaging. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual- monochromator microplate reader platform offering a wide range of. M4 Contracting Ltd, Victoria, British Columbia. normalized response (Graphpad Prism 8. If you need to relocate a SpectraMax Multi-Mode Microplate Reader, follow these steps. The BD plate dimensions are 5. After incubation, the biofilms were washed with PBS until fully remove crystal violet solutions and then, solubilized by adding a 150 μL acetic acid. Steady-state kinetics measurements were performed at 25°C in 96-well plates (Corning) using a SpectraMax M4 spectrophotometer (Molecular Devices) in PBS buffer. We therefore gathered salient information about many of the. The DQ Green BSA probe. Results were normalized and expressed as a percentage of live cells. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. 22 μM was mixed with 10-μl enzyme giving a final enzyme concentration in the. All associated StakMax® stackers. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading 312 gain calibration failed. Transfected cells were seeded into 6-well plates at a density of 200 cells/well. In. FlexStation ®, SpectraMax. Background-subtracted data values (mean +/- SD, n =2) are graphed. After incubating for 10 min at room temperature, the absorbances of standards and samples were measured at 450 and 590 nm, 12 using a Spectramax M4 spectrophotometer (Molecular Devices). CMC values were determined from the pyrene fluorescence in solution as a function of conjugate. Detection modalities are shown in Table 1-1. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. The 96-well plates were read on the SpectraMax M4 plate reader at the following wavelengths: green fluorescence at 488 ex.